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Optimized loop-mediated isothermal amplification assay for Tomato leaf curl New Delhi virus-[potato] detection in potato leaves and tubers

September 19 2017 by INKOA
Resource image of the African potato platform

Apical leaf curl disease of potato is caused by a whitefly transmitted begomovirus, Tomato leaf curl New Delhi virus-[potato] (ToLCNDV-[potato]) in India. Detection of this virus is essential to manage the disease, particularly in healthy potato seed production systems. Large scale testing of micro-plants demands a simple, rapid and sensitive assay. Hence, loop-mediated isothermal amplification (LAMP) method was developed for specific detection of ToLCNDV-[potato]. Six primers that recognize the coat protein gene sequence of ToLCNDV-[potato] were designed and LAMP assay was optimized using different concentrations of magnesium sulphate, betaine, dNTPs, Bst DNA polymerase and temperature. The results were assessed by visual observation of turbidity, colour change using SYBR green dye and also by gel electrophoresis. The assay successfully detected the virus in infected plants collected from potato fields whereas no cross-reactions were observed with healthy plants and other potato viruses. The optimized assay was as sensitive as PCR assay and could detect up to 0.002 pg of total DNA. The assay could detect the virus in infected potato tubers and also in asymptomatic plants. Print-capture LAMP assay was developed and its application could reduce the cost and time of the assay in large scale testing under seed production



Potato Growers’ Biosecurity Manual
July 2 2018 by INKOA

In May 2018 was published Potato Growers’ Biosecurity Manual by the Industry Representative Body for vegetable and potato growers AUSVEG.
The manual is specific to the Australian situation, but all potato growers can find in it interesting information or tips.


E-Agriculture in action: Drones for agriculture
April 10 2018 by INKOA

This publication is the second in the series titled E-agriculture in action (2016), launched by FAO and ITU, and builds on the previous FAO publications that highlight the use of ICT for agriculture such as Mobile technologies for agriculture and rural development (2012), Information and communication technologies for agriculture and rural development (2013) and Success stories on information and communication technologies for agriculture and rural development (2015).

Bringing Climate Smart Agriculture to Scale:Experiences from the Water Productivity Project in East and Central Africa
March 21 2018 by INKOA

Since 2010, six research organizations in the region have implemented a regional project that sought to combat food insecurity, poverty and climate change by up-scaling Climate-Smart Agriculture (CSA) technologies across farms and landscapes using the Climate Smart Landscape (CSL) approach. Several CSA technologies were evaluated and promoted across landscapes using this approach with remarkable success.

Novel Combined Freeze-Drying and Instant Controlled Pressure Drop Drying for Restructured Carrot-Potato Chips: Optimized by Response Surface Method
March 13 2018 by INKOA

Combined freeze-drying and instant controlled pressure drop process (FD-DIC) for restructured carrot-potato chips was developed and its processing conditions were optimized using response surface methodology (RSM) with the purpose of improving the quality of products and reducing energy consumption. Three critical variables including the amount of carrot, the moisture content of the partially dried product before DIC treatment, and equilibrium temperature of DIC for the restructured chips were considered. 

Light interim evaluation of the Africa-EU Research and Innovation partnership on Food and Nutrition Security and Sustainable Agriculture
February 1 2018 by INKOA

This report presents the results of a light interim evaluation of the first 1.5 years of the Africa-EU research and innovation (R&I) partnership on Food and Nutrition Security and Sustainable Agriculture (FNSSA). The evaluation responds to a request from the April 2016 meeting of senior officials of the EU-Africa High Level Policy Dialogue (HLPD) for a report on the progress made in the FNSSA R&I partnership to the November 2017 Africa-EU Summit.

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